OMB APPROVAL OMB Number: 3235-0578 Expires: April 30, 2010 Estimated average burden hours per response..... 10.5 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM N-Q CERTIFIED SHAREHOLDER REPORT OF REGISTERED MANAGEMENT INVESTMENT COMPANIES Investment Company Act file number 811-21409 Pioneer Municipal High Income Advantage Trust (Exact name of registrant as specified in charter) 60 State Street, Boston, MA 02109 (Address of principal executive offices) (ZIP code) Dorothy E. Bourassa, Pioneer Investment Management, Inc., 60 State Street, Boston, MA 02109 (Name and address of agent for service) Registrant's telephone number, including area code: (617) 742-7825 Date of fiscal year end: March 31 Date of reporting period: December 31, 2009 Form N-Q is to be used by management investment companies, other than small business investment companies registered on Form N-5 (239.24 and 274.5 of this chapter), to file reports with the Commission, not later than 60 days after close of the first and third fiscal quarters, pursuant to Rule 30b1-5under the Investment Company Act of 1940 (17 CFR 270.30b-5). The Commission may use the information provided on Form N-Q in its regulatory, disclosure review, inspection, and policymaking roles. A registrant is required to disclose the information specified by Form N-Q, and the Commission will make this information public. A registrant is not required to respond to the collection of information contained in Form N-Q unless the Form displays a currently valid Office of Management and Budget ("OMB") control number. Please direct comments concerning the accuracy of the information collection burden estimate and any suggestions for reducing the burden to Secretary, Securities and Exchange Commission, 450 Fifth Street, NW, Washington, DC 20549-0609. The OMB has reviewed this collection of information under the clearance requirements of 44 U.S.C. ss. 3507. ITEM 1. Schedule of Investments. Pioneer Municipal High Income Advantage Trust SCHEDULE OF INVESTMENTS 12/31/09 (unaudited) Principal Amount Value TAX-EXEMPT OBLIGATIONS - 143.3% of Net Assets Alabama - 0.7% 2,500,000 Huntsville-Redstone Village Special Care Fa $ 1,833,225 Arizona - 3.4% 5,000,000 Casa Grande Industrial Development Authorit $ 4,687,600 1,531,000 Pima County Industrial Development Authorit 1,393,455 2,640,000 Pima County Industrial Development Authorit 2,289,250 1,000,000 San Luis Facility Development Corp., 7.25%, 911,640 $ 9,281,945 California - 12.1% 3,140,000 California State University Revenue, RIB, 1 $ 3,175,576 2,425,000 California State Various Purpose, 5.75%, 4/ 2,449,008 8,575,000 California State Various Purpose, RIB, 10.6 7,384,790 803,175 California Statewide Communities Developmen 160,635 2,680,000 Golden State Tobacco Securitization Corp., 3,145,194 2,500,000 San Jose California Airport Revenue, 5.0%, 2,324,575 10,500,000 University of California, RIB, 10.994%, 5/1 10,363,290 7,670,000 Valley Health System Hospital Revenue, 6.87 4,216,199 $ 33,219,267 Colorado - 1.1% 2,850,000 Denver Health & Hospital Authority Healthca $ 3,130,725 Connecticut - 2.8% 7,750,000 Connecticut State Development Authority Rev $ 7,570,587 District of Columbia - 3.4% 2,700,000 District of Columbia Tobacco Settlement Fin $ 2,623,995 6,825,000 District of Columbia Tobacco Settlement Fin 6,780,637 $ 9,404,632 Florida - 3.8% 4,500,000 Hillsborough County Industrial Development Authority Pollution Control Revenue, 5.5%, 10/1/23 $ 4,547,565 1,980,000 Hillsborough County Industrial Development Authority Pollution Control Revenue, 6.75%, 7/1/29 1,806,532 1,000,000 Hillsborough County Industrial Development 1,105,300 500,000 Miami Beach Health Facilities Authority, 5. 404,810 2,500,000 Miami-Dade County Florida Aviation Revenue, 2,537,475 $ 10,401,682 Georgia - 4.1% 5,210,000 Atlanta Georgia Water and Wastewater Revenu $ 5,160,870 1,000,000 Clayton County Development Authority Specia 996,500 1,065,000 Effingham County Industrial Development Aut 1,048,013 3,360,000 Fulton County Residential Care Facilities, 2,515,397 1,650,000 Savannah Georgia Economic Development Autho 1,620,960 $ 11,341,740 Guam - 1.9% 4,500,000 Northern Mariana Islands, 6.75%, 10/1/33 $ 5,300,955 Idaho - 1.8% 2,000,000 Power County Industrial Development Corp., $ 1,990,440 3,000,000 Power County Pollution Control Revenue, 5.6 2,969,610 $ 4,960,050 Illinois - 6.8% 3,000,000 Centerpoint Intermodal Center, 7.5%, 6/15/2 $ 2,997,330 3,000,000 City of Chicago Illinois, 5.0%, 1/1/28 3,077,520 2,000,000 City of Chicago Illinois, 5.5%, 12/1/30 1,310,000 1,450,000 Illinois Finance Authority Revenue, 5.5%, 4 1,418,912 2,000,000 Illinois Finance Authority Revenue, 6.0%, 8 2,085,900 1,000,000 Illinois Finance Authority Revenue, 6.0%, 1 832,100 2,000,000 Illinois Finance Authority Revenue, 6.0%, 8 2,167,060 2,500,000 Illinois Finance Authority Revenue, 6.125%, 2,237,500 1,500,000 Illinois Health Facilities Authority Revenu 660,750 2,615,000 Southwestern Illinois Development Authority 1,837,561 $ 18,624,633 Indiana - 5.7% 10,000,000 Indiana State Development Finance Authority $ 9,761,100 5,000,000 Jasper County Industrial Economic Developme 4,317,400 1,995,000 Vincennes Industrial Economic Development R 1,641,745 $ 15,720,245 Kentucky - 1.6% 500,000 Kentucky Economic Development Finance Autho $ 499,975 4,400,000 Kentucky Economic Development Finance Autho 3,801,732 $ 4,301,707 Louisiana - 6.3% 2,500,000 Louisiana Local Government Environmental Fa $ 2,538,675 6,000,000 Louisiana Public Facilities Authority Reven 5,449,860 750,000 Opelousas Louisiana General Hospital Author 755,490 9,415,000 Tobacco Settlement Financing Corp., 5.875%, 8,658,693 $ 17,402,718 Maryland - 0.4% 460,000 Maryland Health & Higher Educational Facili $ 372,076 1,250,000 Maryland Health & Higher Educational Facili 865,275 $ 1,237,351 Massachusetts - 6.5% 830,000 Massachusetts Development Finance Agency, 5 $ 717,892 2,500,000 Massachusetts Development Finance Agency, 5 2,485,500 1,955,000 Massachusetts Development Finance Agency, 7 1,520,677 2,195,000 Massachusetts Health & Educational Faciliti 1,872,203 1,000,000 Massachusetts Health & Educational Faciliti 1,138,120 10,760,000 Massachusetts Housing Finance Agency, 5.35% 10,124,084 $ 17,858,476 Michigan - 3.7% 1,500,000 Delta County Michigan Economic Development $ 1,672,980 3,000,000 Macomb County Hospital Finance Authority Re 3,522,840 745,000 Michigan Public Educational Facilities Auth 639,322 4,130,000 Michigan State Hospital Finance Authority R 3,545,811 1,000,000 Michigan State Strategic Fund Solid Waste D 807,060 $ 10,188,013 Missouri - 1.5% 1,850,000 Kansas City Industrial Development Authorit $ 1,442,760 1,500,000 St. Louis Industrial Development Authority 509,640 6,640,000 St. Louis Industrial Development Authority 2,255,143 $ 4,207,543 Montana - 0.5% 2,445,000 Hardin Increment Industrial Infrastructure $ 1,226,632 1,000,000 Two Rivers Authority, Inc., Project Revenue 154,090 $ 1,380,722 Nevada - 2.3% 1,500,000 Henderson Nevada Health Care Facilities Rev $ 1,541,010 1,600,000 Nevada State Department of Business & Indus 80,000 1,000,000 Nevada State Department of Business & Indus 50,000 1,320,000 Nevada State Department of Business & Indus 66,000 5,000,000 Reno Nevada Hospital Revenue, 5.25%, 6/1/41 4,491,700 $ 6,228,710 New Hampshire - 0.4% 1,125,000 New Hampshire Health & Educational Faciliti $ 1,049,805 New Jersey - 12.1% 2,500,000 Burlington County New Jersey Bridge Commiss $ 2,101,800 13,000,000 New Jersey Economic Development Authority R 11,208,080 3,500,000 New Jersey State Turnpike Authority, RIB, 1 4,909,065 10,375,000 New Jersey Transportation Trust Fund Author 4,127,382 1,000,000 Tobacco Settlement Financing Corp., 6.25%, 1,168,180 3,250,000 Tobacco Settlement Financing Corp., 6.75%, 3,850,795 5,000,000 Tobacco Settlement Financing Corp., 7.0%, 6 5,964,150 $ 33,329,452 New York - 6.5% 1,900,000 Dutchess County Industrial Development Agen $ 1,858,238 1,000,000 Nassau County New York Industrial Developme 889,500 5,000,000 New York City Industrial Development Agency 3,349,400 3,950,000 New York City Industrial Development Agency 3,599,635 5,000,000 New York State Environmental Facilities Cor 5,124,900 3,000,000 Ulster County New York Industrial Developme 2,692,200 500,000 Ulster County New York Industrial Developme 396,985 $ 17,910,858 North Carolina - 1.9% 6,000,000 Charlotte North Carolina Special Facilities $ 5,136,900 Ohio - 1.9% 2,375,000 Belmont County Health System Revenue, 5.7%, $ 2,210,983 1,000,000 Belmont County Health System Revenue, 5.8%, 806,640 3,000,000 Cleveland Airport Special Revenue, 5.375%, 2,256,090 $ 5,273,713 Oklahoma - 2.8% 1,500,000 Jackson County Memorial Hospital Authority $ 1,500,780 2,220,000 Tulsa Municipal Airport Trust Revenue, 6.25 1,877,698 1,500,000 Tulsa Municipal Airport Trust Revenue, 7.35 1,482,585 3,000,000 Tulsa Municipal Airport Trust Revenue, 7.75 2,938,860 $ 7,799,923 Pennsylvania - 10.0% 3,000,000 Allegheny County Hospital Development Autho $ 3,291,270 500,000 Allegheny County Hospital Development Autho 548,545 1,000,000 Clarion County Hospital Authority Revenue, 1,001,010 3,600,000 Columbia County Hospital Authority Revenue, 3,175,560 945,000 Hazleton Health Services Authority Hospital 897,211 1,405,000 Montgomery County Higher Education & Health 1,410,030 1,430,000 Pennsylvania Economic Development Financing 1,270,555 1,805,000 Pennsylvania Economic Development Financing 1,789,585 2,330,000 Pennsylvania Economic Development Financing 2,266,671 5,000,000 Pennsylvania Economic Development Financing 3,913,500 5,000,000 Philadelphia Hospitals & Higher Education F 3,807,650 2,005,000 Scranton-Lackawanna Health & Welfare Author 1,961,973 2,245,000 Scranton-Lackawanna Health & Welfare Author 2,167,660 $ 27,501,220 Rhode Island - 3.2% 1,385,000 Central Falls Rhode Island Detention Facili $ 1,179,078 8,285,000 Tobacco Settlement Financing Corp., 6.25%, 7,690,965 $ 8,870,043 South Carolina - 6.8% 1,500,000 Connector 2000 Association, Inc., Toll Road $ 360,120 7,140,000 South Carolina Jobs Economic Development Au 8,332,380 860,000 South Carolina Jobs Economic Development Au 1,011,188 3,000,000 South Carolina Jobs Economic Development Au 3,216,000 4,400,000 Tobacco Settlement Revenue Management, 6.37 5,688,452 $ 18,608,140 Tennessee - 3.8% 1,000,000 Johnson City Health & Educational Facilitie $ 1,132,060 7,000,000 Knox County Health Educational & Housing Fa 7,346,710 2,400,000 Sullivan County Health Educational and Hous 2,006,208 $ 10,484,978 Texas - 17.5% 8,650,000 Brazos River Authority Pollution Control Re $ 4,600,330 2,827,399 Gulf Coast Industrial Development Authority 565,480 10,000,000 Houston Texas Airport System Special Facili 9,315,100 960,000 IAH Public Facility Corp., Project Revenue 878,736 1,000,000 IAH Public Facility Corp., Project Revenue 823,410 1,350,000 IAH Public Facility Corp., Project Revenue 1,057,023 2,750,000 Lower Colorado River Authority, 5.0%, 5/15/ 2,776,153 845,000 Lubbock Health Facilities Development Corp. 756,233 2,000,000 Lubbock Health Facilities Development Corp. 1,748,980 7,750,000 Matagorda County Navigation District Number 7,435,505 9,750,000 North Texas Tollway Authority Revenue, 5.75 9,994,725 1,711,000 Panhandle Regional Housing Finance Corp. Mu 1,803,291 1,000,000 Richardson Hospital Authority Revenue, 6.0% 865,990 1,000,000 Texas Midwest Public Facility Corp. Revenue 1,032,020 4,700,000 Tomball Hospital Authority, 6.0%, 7/1/25 4,463,825 $ 48,116,801 Utah - 0.5% 1,600,000 Spanish Fork Charter School Revenue, 5.7%, $ 1,255,248 Virginia - 0.8% 2,000,000 Washington County Industrial Development Au $ 2,282,520 Washington - 3.3% 1,195,000 Tobacco Settlement Authority Revenue, 6.5%, $ 1,201,871 2,000,000 Washington State Health Care Facilities Aut 2,010,100 2,000,000 Washington State Health Care Facilities Aut 2,017,900 5,000,000 Washington State Housing Finance Commission 3,778,850 $ 9,008,721 Wisconsin - 1.4% 2,900,000 Aztalan Wisconsin Exempt Facilities Revenue $ 404,550 1,000,000 Wisconsin State Health & Educational Facili 999,410 1,000,000 Wisconsin State Health & Educational Facili 948,580 1,500,000 Wisconsin State Health & Educational Facili 1,612,980 $ 3,965,520 TOTAL TAX-EXEMPT OBLIGATIONS (Cost $400,789,360) $ 394,188,768 MUNICIPAL COLLATERALIZED DEBT OBLIGATION - 3.6% of Net Assets 13,000,000 Non-Profit Preferred Funding Trust I, Serie $ 9,807,330 TOTAL MUNICIPAL COLLATERALIZED DEBT OBLIGATION (Cost $13,000,000) $ 9,807,330 Shares COMMON STOCK - 0.5% of Net Assets 109,625 Delta Airlines, Inc. $ 1,247,533 TOTAL COMMON STOCK (Cost $3,210,349) $ 1,247,533 TAX-EXEMPT MONEY MARKET MUTUAL FUND - 2.9% of Net Assets 8,000,000 BlackRock Liquidity Funds MuniFund Portfoli $ 8,000,000 TOTAL TAX-EXEMPT MONEY MARKET MUTUAL FUND (Cost $8,000,000) $ 8,000,000 TOTAL INVESTMENTS IN SECURITIES - 150.3% (Cost $424,999,709) (h)(i) $ 413,243,631 OTHER ASSETS AND LIABILITIES 4.3% $ 11,752,009 PREFERRED SHARES AT REDEMPTION VALUE, INCLUDING DIVIDENDS PAYABLE - (54.6)% $ (150,007,899) NET ASSETS APPLICABLE TO COMMON SHAREOWNERS $ 274,987,741 (144A) Security is exempt from registration under Rule 144A of the Securities Act of 1933. Such securities may be resold normally to qualified institutional buyers in a transaction exempt from registration. At December 31, 2009, the value of these securities amounted to $36,132,569, or 13.1% of total net assets applicable to common shareowners. RIB Residual Interest Bonds. + Prerefunded bonds have been collateralized by U.S. Treasury or U.S. Government Agency securities which are held in escrow to pay interest and principal on the tax exempt issue and to retire the bonds in full at the earliest refunding date. (a) The interest rate is subject to change periodically and inversely based upon prevailing market rates. The rate shown is the coupon rate at December 31, 2009. (b) The interest rate is subject to change periodically. The interest rate shown is the rate at December 31, 2009. (c) Security is in default and is non-income producing. (d) Debt obligation initially issued at one coupon which converts to a higher coupon at a specific date. The rate shown is the coupon rate at December 31, 2009. (e) Escrow to maturity. (f) Indicates a security that has been deemed illiquid. The aggregate cost of illiquid securities is $13,000,000. The aggregate value is $8,953,490, or 3.9% of the total net assets applied to common shareowners. (g) Non-income producing. (i) At December 31, 2009, the net unrealized loss on investments based on cost for federal income tax purposes of $420,011,921 was as follows: Aggregate gross unrealized gain for all investments in which there is an excess of value over tax cost $33,945,811 Aggregate gross unrealized loss for all investments in which there is an excess of tax cost over value (40,714,101) Net unrealized loss ($6,768,290) For financial reporting purposes net unrealized loss on investments was $11,756,078 and cost of investments aggregated $424,999,709. Various inputs are used in determining the value of the Trust's investments. These inputs are summarized in the three broad levels listed below. Highest priority is given to Level 1 inputs and lowest priority is given to Level 3. Level 1 - quoted prices in active markets for identical securities Level 2 - other significant observable inputs (including quoted prices for similar securities, interest rates, prepayment speeds, credit risk, etc.) Level 3 - significant unobservable inputs (including the Trust's own assumptions in determining fair value of investments) The following is a summary of the inputs used as of December 31, 2009, in valuing the Trust's assets: Level 1 Level 2 Level 3 Total Tax exempt obligations $0 $394,188,768 $0 $394,188,768 Municipal collateralized d 0 9,807,330 0 9,807,330 Common stock 1,247,533 0 0 1,247,533 Tax-exempt money market 8,000,000 0 0 8,000,000 Total $9,247,533 $403,996,098 $0 $413,243,631 ITEM 2. CONTROLS AND PROCEDURES. (a) Disclose the conclusions of the registrant's principal executive officer or officers and principal financial officer or officers, or persons performing similar functions, about the effectiveness of the registrant's disclosure controls and procedures (as defined in Rule 30a-3(c) under the Act (17 CFR 270.30a-3(c))) as of a date within 90 days of the filing date of the report that includes the disclosure required by this paragraph, based on their evaluation of these controls and procedures required by Rule 30a-3(b) under the Act (17 CFR270.30a-3(b))) and Rule 13a-15(b) or 15d-15(b) under the Exchange Act (17 CFR240.13a-15(b) or 240.15d-15(b)). The registrant's principal executive officer and principal financial officer have concluded that the registrant's disclosure controls and procedures are effective based on their evaluation of these controls and procedures as of a date within 90 days of the filing date of this report. (b) Disclose any change in the registrant's internal controls over financial reporting (as defined in Rule 30a-3(d) under the Act (17 CFR270.30a-3(d)) that occurred during the registrant's last fiscal quarter that has materially affected, or is reasonably likely to materially affect, the registrant's internal control over financial reporting. There were no significant changes in the registrant's internal control over financial reporting that occurred during the second fiscal quarter of the period covered by this report that have materially affected, or are reasonably likely to materially affect, the registrant's internal control over financial reporting. ITEM 3. EXHIBITS. (a) A separate certification for each principal executive officer and principal financial officer of the registrant as required by Rule 30a-2 under the Act (17 CFR 270.30a-2). Filed herewith. SIGNATURES [See General Instruction F] Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, the registrant has duly caused this report to be signed on its behalf by the undersigned, thereunto duly authorized. (Registrant) Pioneer Municipal High Income Advantage Trust By (Signature and Title)* /s/ John F. Cogan, Jr. John F. Cogan, Jr, President Date March 1, 2010 Pursuant to the requirements of the Securities Exchange Act of 1934 and the Investment Company Act of 1940, this report has been signed below by the following persons on behalf of the registrant and in the capacities and on the dates indicated. By (Signature and Title)* /s/ John F. Cogan, Jr. John F. Cogan, Jr., President Date March1 , 2010 By (Signature and Title)* /s/ Mark Bradley Mark Bradley, Treasurer Date March 1, 2010 * Print the name and title of each signing officer under his or her signature.